FIG 1.

Wild-type and modified NSP3 proteins encoded by pT7 plasmids and rotaviruses. (A) Organization of pT7 plasmids expressing wild-type NSP3 and NSP3-FL/UnaG (+)RNAs, indicating the locations of the T7 promoter (prm) and the hepatitis delta virus (HDV) self-cleaving ribozyme (Rz). Nucleotide positions are labeled. (B) Products of recombinant RVAs expressing wild-type NSP3 and NSP3-FL-UnaG and RVC (Bristol strain) expressing NSP3-2A-dsRBP, including approximate locations of functional domains in NSP3 (21, 22). The red arrow indicates the position of the stop-restart cleavage site in the 2A-like element in the RVC NSP3-2A-dsRBP ORF. Amino acid positions are labeled. FL represents a 3× Flag tag.