FIG 7.

pT7 plasmids and rotaviruses expressing NSP1. (A) Organization of pT7 plasmids expressing wild-type NSP1 and NSP1-FL-UnaG (+)RNAs, indicating the locations of the T7 promoter (prm) and the hepatitis delta virus (HDV) self-cleaving ribozyme (Rz). The red arrow notes the position of the stop codon in the NSP1-FL-UnaG ORF. (B) Products of recombinant RVAs expressing wild-type NSP1 and NSP1-FL-UnaG. (C) Profiles of the 11 genomic dsRNAs recovered from rSA11 viruses, resolved by PAGE. The red arrowheads note the position of the segment 5 dsRNA. wt, wild-type. (D) Western blot analysis of proteins present at 8 hpi in MA104 cells infected with recombinant viruses.