Fig. 7.
Effect of acrylammide on the fluorescence emission of EST2 (closed symbols) and AFEST(opened symbols) at different temperatures. Acrylammide quenching of NATA at 25°C was adopted from Eftink and Ghiron,34 and data for AFEST were adopted from D’Auria et al.19 The Stern-Volmer plots were calculated according to Eftink.42