TABLE I.

Fluorescence Anisotropy of the EST2 at Different Temperatures

t (C°)	θk (ns)	δθk (ns)a	gkc	δgka	${\chi }_{\text{R}}^2$b
25.0	0.01	0.01	0.26	0.01
	16.5	−0.6, +0.7	0.74	0.01	1.8
45.0	0.02	−0.01,+0.006	0.28
	9.8	−0.2, +0.4	0.72	−0.01,+0.007	1.4
65.0	0.01	−0.01,+0.004	0.29	0.01
	5.5	0.2	0.71	0.01	1.3

Protein concentration 0.05 mg/ml in 50 mM Tris-HCl, 2.5 mM MgCl₂, 0.5 mM EDTA, 1 mM DTT buffer, pH 7.1.

^aStandard deviations calculated by the Monte Carlo method.

^bFor experimental uncertainties δp = 0.2 deg and δm = 0.005.

^cFor λexc = 298 nm the r₀ was fixed to the value of 0.29.⁴²