Table 2.
List of all redox proteins detected, the redox state of selected redox Cys residues labelled with both d(0) NEM and d(5) NEM was calculated in Skyline using the ratio of the average ion intensity of parent ions. The m/z values and retention times of selected peptides were applied in the targeted approach using Skyline open software. Proteins in bold indicate that there were significant differences in abundance between young and very old samples.
| Accession | Protein | Redox cys | Adult red/ox | old red/ox | Adult vs. old | (−)10logP |
|---|---|---|---|---|---|---|
| P63101 | 14-3-3 protein zeta/delta (Ywhaz) | 94 | 12.94 | 13.11 | 1.00:1.41 | 19.28 |
| 189 | 6.62 | 4.97 | ||||
| P61982 | 14-3-3 protein gamma (Ywhag) | 112 | 20.82 | 20.27 | 1.00:1.20 | 3.97 |
| P60597 | 2-oxoglutarate dehydrogenase, mitochondrial (Ogdh) | 395 | 25.75 | 22.71 | 1.00:0.92 | 4.1 |
| 565 | 12.64 | 13.41 | ||||
| 904 | 6.74 | 4.44 | ||||
| Q99KI0 | Aconitate hydratase, mitochondrial (Aco2) | 126 | 6.36 | 7.22 | 1.00:1.16 | 5.29 |
| 385 | 2.03 | 2.27 | ||||
| 592 | 1.99 | 2.02 | ||||
| P68134 | Actin, alpha skeletal muscle (Acta1) | 219 | 8.82 | 8.22 | 1.00:1.01 | 0.36 |
| 259 | 3.67 | 3.67 | ||||
| 287 | 8.14 | 8.26 | ||||
| P48962 | ADP/ATP translocase 1 (Slc25a4) | 160 | 32.43 | 37.85 | 1.00:0.90 | 5.37 |
| P47738 | Aldehyde dehydrogenase, mitochondrial (Aldh2) | 388 | 17.60 | 16.17 | 1.00:1.17 | 5.22 |
| P45376 | Aldose reductase (Akr1b1) | 200 | 10.86 | 14.10 | 1.00:0.86 | 2.96 |
| 289/94 | 4.18 | 5.35 | ||||
| Q61838 | Alpha-2-macroglobulin (A2m) | 340 | 5.39 | 6.05 | 1.00:1.57 | 25.88 |
| P05201 | Aspartate aminotransferase, cytoplasmic (Got1) | 391 | 11.45 | 12.53 | 1.00:1.12 | 8.04 |
| P05202 | Aspartate aminotransferase, mitochondrial (Got2) | 106 | 43.21 | 40.70 | 1.00:1.10 | 4.48 |
| 187 | 10.14 | 8.45 | ||||
| 295 | 8.99 | 11.51 | ||||
| Q9DCX2 | ATP synthase subunit d mitochondrial (Atp5h) | 101 | 3.72 | 3.87 | 1.00:0.97 | 2.82 |
| P56382 | ATP synthase subunit epsilon, mitochondrial (Atp5e) | 19 | 13.95 | 14.19 | 1.00:1.18 | 5.67 |
| Q9CZU6 | Citrate synthase, mitochondrial (Cs) | 359 | 8.92 | 8.10 | 1.00:0.72 | 27.95 |
| P18760 | Cofilin-1 (Cfl1) | 147 | 7.23 | 5.26 | 1.00:1.72 | 31.7 |
| Q6P8J7 | Creatine kinase S-type, mitochondrial (Ckmt2) | 317 | 30.69 | 30.36 | 1.00:0.64 | 16.04 |
| P07310 | Creatine kinase M-type (Ckm) | 254 | 54.98 | 46.91 | 1.00:0.63 | 68.46 |
| Q9CV13 | Cytochrome b-c1 complex subunit 1, mitochondrial (Uqcrc1) | 380 | 7.59 | 8.08 | 1.00:1.05 | 2.35 |
| P99028 | Cytochrome b-c1 complex subunit 6, mitochondrial (Uqcrh) | 51 | 0.12 | 0.11 | 1.00:1.12 | 3.6 |
| O35215 | D-dopachrome decarboxylase (Ddt) | 57 | 0.51 | 0.56 | 1.00:1.12 | 2.87 |
| Q99LC5 | Electron transfer flavoprotein subunit alpha, mitochondrial (Etfa) | 53 | 4.73 | 3.90 | 1.00:1.00 | 1.75 |
| P1782/P21580 | Alpha-enolase (Eno1) | 337 | 2.48 | 2.58 | 1.00:0.90 | 7.87 |
| 357 | 9.41 | 11.12 | ||||
| 389 | 9.74 | 11.73 | ||||
| 399 | 0.65 | 0.78 | ||||
| P04117 | Fatty acid-binding protein, adipocyte (Fabp4) | 118 | 7.54 | 8.95 | 1.00:1.15 | 5.94 |
| P97447 | Four and a half LIM domains protein 1 (Fhl1 ) | 71 | 7.44 | 7.15 | 1.00:0.62 | 43.48 |
| 89 | 6.50 | 6.54 | ||||
| 255 | 1.36 | 2.09 | ||||
| 273/76 | 1.77 | 2.26 | ||||
| P05064 | Fructose-bisphosphate aldolase A (Aldoa) | 178 | 2.06 | 1.35 | 1.00:0.58 | 62.34 |
| 339 | 9.51 | 9.91 | ||||
| P16858 | Glyceraldehyde-3-phosphate dehydrogenase (Gapdh) | 22 | 12.81 | 12.05 | 1.00:0.60 | 63.76 |
| 150/54 | 1164.5 | 471.3 | ||||
| Q9WUB3 | Glycogen phosphorylase, muscle form (Pygm) | 172 | 11.36 | 12.34 | 1.00:0.69 | 33.84 |
| P15626 | Glutathione S-transferase Mu 2 (Gstm2) | 87 | 0.53 | 0.88 | 1.00:1.46 | 14.86 |
| P63017 | Heat shock cognate 71 kDa protein (Hspa8) | 603 | 7.52 | 6.29 | 1.00:0.94 | 1.8 |
| P54071 | Isocitrate dehydrogenase [NADP], mitochondrial (Idh2) | 113 | 0.11 | 0.09 | 1.00:1.07 | 5.04 |
| 308 | 59.88 | 45.45 | ||||
| 392 | 9.37 | 9.15 | ||||
| P70404 | Isocitrate dehydrogenase [NAD] subunit gamma 1, mitochondrial (Idh3g) | 235/36 | 25.03 | 18.14 | 1.00:0.79 | 11.21 |
| Q9JHI5 | Isovaleryl-CoA dehydrogenase, mitochondrial (Ivd) | 252 | 8.62 | 6.53 | 1.00:1.20 | 5.66 |
| P06151 | L-lactate dehydrogenase A chain (Ldha) | 84 | 16.84 | 15.84 | 1.00:0.73 | 20.17 |
| P16125 | & LDHB | 163 | 11.73 | 11.83 | 1.00:0.92 | 3.58 |
| P51174 | Long-chain specific acyl-CoA dehydrogenase, mitochondrial (Acadl) | 166 | 5.74 | 5.65 | 1.00:1.24 | 14.76 |
| 342 | 9.36 | 11.79 | ||||
| 351 | 4.41 | 5.32 | ||||
| P08249 | Malate dehydrogenase, mitochondrial (Mdh2) | 93 | 6.69 | 7.62 | 1.00:1.06 | 4.34 |
| 212 | 10.73 | 9.67 | ||||
| 285 | 7.83 | 10.69 | ||||
| P14152 | Malate dehydrogenase, cytoplasmic (Mdh1) | 137 | 9.61 | 9.88 | 1.00:0.94 | 3.84 |
| 154 | 9.36 | 9.86 | ||||
| P04247 | Myoglobin (Mb) | 67 | 48.08 | 51.68 | 1.00:1.02 | 1.05 |
| P97457 | Myosin regulatory light chain 2, skeletal muscle isoform (Mylpf) | 128 | 37.29 | 37.72 | 1.00:01.04 | 1.3 |
| 157 | 25.39 | 20.91 | ||||
| Q01768 | Nucleoside diphosphate kinase B (Nme2) | 109 | 2.88 | 2.06 | 1.00:0.69 | 17.23 |
| P99029 | Peroxiredoxin-5, mitochondrial (Prdx5) | 96 | 10.50 | 7.98 | 1.00:1.05 | 2.1 |
| O08709 | Peroxiredoxin-6 (Prdx6) | 47 | 0.96 | 1.60 | 1.00:0.90 | 2.88 |
| O70250 | Phosphoglycerate mutase 2 (Pgam2) | 23 | 11.53 | 10.69 | 1.00:0.67 | 39.65 |
| 153 | 21.41 | 20.78 | ||||
| Q9D0F9 | Phosphoglucomutase-1 (Pgm1) | 374 | 22.25 | 23.58 | 1.00:0.71 | 21.73 |
| Q9QYG0 | Protein NDRG2 (Ndrg2) | 255 | 39.33 | 10.47 | 1.00:0.94 | 5.1 |
| P35486 | Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial (Pdha1) | 41 | 3.81 | 3.11 | 1.00:0.80 | 11.94 |
| Q9D051 | Pyruvate dehydrogenase E1 component subunit beta, mitochondrial (Pdhb) | 263 | 26.22 | 27.19 | 1.00:0.87 | 7.74 |
| P52480 | Pyruvate kinase PKM (Pkm) | 49 | 16.40 | 17.90 | 1.00:0.69 | 33.86 |
| 152 | 7.66 | 7.88 | ||||
| 358 | 14.78 | 13.25 | ||||
| 474 | 70.16 | 68.01 | ||||
| Q8R429 | Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 (Atp2a1) | 674/75 | 40.53 | 52.44 | 1.00:0.66 | 37.47 |
| P07759 | Serine protease inhibitor A3K (Serpina3k) | 260 | 8.63 | 18.18 | 1.00:0.52 | 60.81 |
| Q921I1 | Serotransferrin (Tf) | 156 | 0.031 | 0.023 | 1.00:2.97 | 89.76 |
| P07724 | Serum albumin (Alb) | 58 | 2.83 | 2.16 | 1.00:1.28 | 28.13 |
| 77 | 1.38 | 1.02 | ||||
| 289 | 0.012 | 0.012 | ||||
| Q9Z2I9 | Succinyl-CoA ligase [ADP-forming] subunit beta, mitochondrial (Sucla2) | 152 | 0.90 | 0.84 | 1.00:0.70 | 17.35 |
| Q9D0K2 | Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial (Oxct1) | 456 | 6.29 | 6.61 | 1.00:1.09 | 2.96 |
| P08228 | Superoxide dismutase [Cu-Zn] (Sod1) | 7 | 0.95 | 0.94 | 1.00:1.06 | 2.54 |
| 147 | 0.65 | 0.56 | ||||
| P17751 | Triosephosphate isomerase (Tpi1) | 117 | 13.52 | 14.21 | 1.00:0.95 | 1.43 |
| 268 | 15.57 | 15.53 | ||||
| P58774 | Tropomyosin beta chain (Tpm2) | 190 | 8.59 | 10.17 | 1.00:1.21 | 6.41 |
| P13412 | Troponin I, fast skeletal muscle (Tnni2) | 134 | 8.94 | 8.90 | 1.00:1.14 | 6.76 |