Fig. 4.
PhoP regulates the transcription of bldD in S. erythraea. a Electrophoretic mobility shift assays of His-PhoP to promoter region of bldD by incubating the bio-labeled DNA with 1 µM protein and a 200-fold excess of nonspecific competitor DNA (sperm DNA). PbldD represented bio-labeled DNA sequence located in region from − 300 to + 50 of bldD gene. b The footprinting assay for promoter region of bldD with PhoP, and the pink solid rectangle refers to region protected by PhoP. Genetic organization of bldD and regulatory sequence were shown. The underline referred to the potential binding sites of BldD and PhoP. The consensus sequences of BldD binding motif in S. erythraea and S. coelicolor were shown inside the gray frame. c The transcriptional profiles of bldD gene in WT, glnR-deleted mutant (ΔglnR) and PhoP-overexpressed strain (OphoP). The transcriptional responses of bldD gene to high (MG-P+) and low (MG-P−) phosphate was also shown