Fig. 7.

LC3B/PINK1 dependent mitophagy levels in IPF AMs. Protein levels of PINK1 (a) and TOMM20 (b) in BAL cells treated with Chloroquine (CQ) for 1 h relative to untreated (NT) from IPF (n = 12 and n = 8 respectively) and control (n = 6 and n = 5 respectively). c Representative Western Blots of control and IPF samples. d and e TOMM20 mean fluorescence intensity/cell measured from images acquired by confocal microscopy, per patient and group respectively. f TOMM20-LC3B II Pearson coefficient of colocalization, Costes’s threshold corrected Pearson coefficient of colocalization and M1 and M2 Menders colocalization values comparisons of IPF and control groups following CQ treatment. For all comparisons Mann-Whitney test was used except for colocalizations where t-test was used. Data are represented as median with interquartile ranges. See methods for Western blot and immune fluorescence analyses