Figure 6.

Both OT D1 and D2 MSNs receive monosynaptic and polysynaptic input from the aPCX. A, Schematic representing light stimulation of PCX neurons during ex vivo patch-clamp recordings of ChR2 infected aPCX neurons. B, An infected pyramidal neuron (red) in the aPCX displayed robust responses to blue light stimulation (1 ms pulses indicated by blue bars). Baseline membrane potential = −50 mV. C, Schematic of the ex vivo whole-cell patch-clamp recording of OT D1 or D2 MSNs and stimulation of aPCX association fibers in the OT. D, A D1 MSN in the OT showing light induced EPSPs to different pulse lengths (top) and to a train of 10 Hz stimuli (bottom) under current-clamp mode. Baseline membrane potential = −70 mV. E, Light-evoked monosynaptic or polysynaptic EPSCs in D1 and D2 MSNs under voltage-clamp mode. The holding potential was −70 mV. F, Summary of D1 and D2 cells that displayed light-evoked synaptic responses, organized by whether the evoked response was defined as monosynaptic or polysynaptic (see Materials and Methods). Values in parentheses indicate the number of cells.