Figure 2.

Estradiol alters action potential properties in arcuate kisspeptin neurons. A, Representative current-clamp recordings of kisspeptin neurons from OVX (left) and OVX+E (right) mice. Current steps (5 pA increments) were applied until the cell initiated firing; action potential properties of the first spike were characterized. B, Expanded axis view of the onset of the spikes in A; arrows mark threshold and spikes were truncated. C–J, Individual values and mean ± SEM values of action potential properties of cells from OVX (O; white circles) and OVX+E (O+E; black circles) mice; C, minimum current to induce firing (rheobase); D, latency; E, action potential threshold; F, action potential rate of rise; G, action potential amplitude; H, FWHM of action potential spike; I, time of AHP; J, AHP amplitude (amp). *p < 0.05 two-tailed unpaired Mann–Whitney U test (C, D, F) or two-tailed unpaired Student's t test (E, G, J). Note some graphs do not include the origin. Mann–Whitney U test did not detect differences between groups in H and I (both p > 0.15).