Figure 2.
PKCα or PKCβ knock-out inhibits slow endocytosis at calyces. A, Sampled ICa (left) and Cm (right) induced by depol20ms (arrow) in a WT calyx. The red curve is a mono-exponential fit of the Cm decay with a τ of 19.1 s. (B) ICa (mean + SEM) and Cm (mean + SEM) induced by depol20ms (arrow) in WT (black, 9 calyces, 9 mice, abbreviated as 9c/9m), PKCα−/− (14c/14m, red) and PKCβ−/− (6c/6m, green) calyces. Data from P7–P10 mice at 22–24°C; SEM plotted every 2 ms for ICa and 1 s for Cm (applies to all similar graphs). C, Ratedecay, the capacitance jump (ΔCm) and the ICa charge (QICa) induced by depol20ms in WT (9c/9m), PKCα−/− (14c/14m) and PKCβ−/− (6c/6m) calyces (P7–P10, 22–24°C). Bar graphs are plotted as mean + SEM (applies to all bar graphs) *p < 0.05; **p < 0.01; t test compared with WT (applies to all bar graphs). D, E, Similar to B and C, respectively, except that the stimulus was 20 APe at 100 Hz (WT, 8c/8m; PKCα−/−, 10c/10m; PKCβ−/−, 8c/8m; P7–P10, 22–24°C). F, G, Cm and Ratedecay (mean + SEM) induced by depol20ms (arrow) in WT (black, 7c/7m) and PKCα−/− calyces at 34–37°C (F; WT, 7c/7m; PKCα−/−, 4c/4m; P7–P10 mice) or at P13–P14 mice (G: WT, 7c/7m; PKCα−/−, 9c/9m; 22–24°C). (H) Cm and Ratedecay (mean + SEM) induced by depol20ms in control (Ctrl, 11c/11m) or in the presence of BIS (11c/11m) or PMA (8c/8m) at 22–24°C in P7–P10 mice.