Table 1.
SK2 deletion affects hippocampal S1P and sphingosine content
| Mean ± SEM (pmol lipid/mg tissue) |
ANOVA results |
|||||
|---|---|---|---|---|---|---|
| WT | SK2Δ | J20 | J20.SK2Δ | F | p | |
| Sphingosine | 1.79 ± 0.21 | 3.69 ± 0.54** | 1.72 ± 0.26 | 3.47 ± 0.31** | 8.86 | 0.0006# |
| S1P | 1.19 ± 0.29 | 0.20 ± 0.05** | 1.18 ± 0.11 | 0.24 ± 0.05** | 11.8 | 0.0001# |
| Ceramide | ||||||
| C16:0 | 1.03 ± 0.11 | 1.16 ± 0.19 | 1.39 ± 0.33 | 1.06 ± 0.09 | 0.64 | 0.60 |
| C18:0 | 70.5 ± 7.0 | 93.5 ± 16.8 | 51.1 ± 10.5 | 86.3 ± 10.5 | 2.55 | 0.084 |
| C20:0 | 0.29 ± 0.03 | 0.27 ± 0.05 | 0.22 ± 0.05 | 0.24 ± 0.03 | 0.57 | 0.64 |
| C22:0 | 0.23 ± 0.04 | 0.15 ± 0.02 | 0.18 ± 0.04 | 0.15 ± 0.01 | 1.79 | 0.18 |
| C22:1 | 0.41 ± 0.13 | 0.21 ± 0.04 | 0.31 ± 0.07 | 0.22 ± 0.02 | 1.44 | 0.26 |
| C24:0 | 0.25 ± 0.07 | 0.09 ± 0.01* | 0.11 ± 0.02* | 0.06 ± 0.01** | 4.84 | 0.011# |
| C24:1 | 1.80 ± 0.52 | 1.05 ± 0.20 | 1.36 ± 0.36 | 0.71 ± 0.05 | 1.92 | 0.16 |
| Total | 74.5 ± 7.23 | 96.4 ± 17.3 | 54.7 ± 11.2 | 88.8 ± 10.7 | 2.28 | 0.12 |
| GalCer | ||||||
| C16:0 | 1.17 ± 0.09 | 1.08 ± 0.26 | 1.68 ± 0.29 | 1.22 ± 0.11 | 1.7 | 0.20 |
| C18:0 | 13.3 ± 1.59 | 16.2 ± 4.77 | 11.7 ± 1.46 | 10.5 ± 0.85 | 0.87 | 0.47 |
| C20:0 | 3.07 ± 0.48 | 3.52 ± 1.27 | 3.13 ± 0.50 | 2.21 ± 0.22 | 0.56 | 0.65 |
| C22:0 | 8.38 ± 1.15 | 7.29 ± 1.93 | 8.04 ± 1.12 | 4.71 ± 0.58 | 1.65 | 0.21 |
| C22:0-OH | 35.1 ± 3.06 | 29.8 ± 6.08 | 31.8 ± 5.12 | 20.9 ± 2.75 | 1.85 | 0.17 |
| C22:1 | 9.71 ± 0.80 | 9.26 ± 2.17 | 9.6 ± 1.31 | 7.06 ± 0.46 | 0.86 | 0.48 |
| C22:1-OH | 3.66 ± 0.27 | 4.18 ± 0.85 | 3.68 ± 0.66 | 3.17 ± 0.34 | 0.51 | 0.68 |
| C24:0 | 9.04 ± 1.29 | 6.23 ± 1.90 | 7.62 ± 1.63 | 3.47 ± 0.44 | 2.79 | 0.067 |
| C24:0-OH | 13.6 ± 1.55 | 11.3 ± 2.01 | 13.2 ± 1.89 | 8.95 ± 1.46 | 1.50 | 0.24 |
| C24:1 | 73.6 ± 9.68 | 61.3 ± 15.9 | 55.9 ± 8.20 | 38.3 ± 3.20 | 2.03 | 0.14 |
| C24:1-OH | 20.2 ± 1.68 | 16.5 ± 4.04 | 16.8 ± 3.38 | 11.6 ± 1.06 | 1.58 | 0.23 |
| Total | 191 ± 20.4 | 167 ± 39.5 | 163 ± 24.0 | 112 ± 8.60 | 1.67 | 0.21 |
| SM | ||||||
| C16:0 | 101 ± 7.45 | 79.5 ± 17.4 | 134 ± 36.3 | 66.4 ± 6.36 | 2.02 | 0.14 |
| C18:0 | 812 ± 126 | 722 ± 88.2 | 583 ± 100 | 463 ± 59.7 | 2.53 | 0.087 |
| C20:0 | 35.3 ± 2.91 | 33.6 ± 6.28 | 36.1 ± 5.90 | 22.3 ± 3.92 | 1.71 | 0.20 |
| C22:0 | 5.16 ± 0.61 | 6.52 ± 1.39 | 9.79 ± 1.58* | 5.00 ± 0.78 | 3.65 | 0.030# |
| C24:0 | 1.99 ± 0.20 | 2.31 ± 0.42 | 3.10 ± 0.72 | 1.71 ± 0.20 | 1.86 | 0.17 |
| C24:1 | 17.8 ± 2.57 | 19.4 ± 3.67 | 19.4 ± 4.04 | 15.0 ± 1.31 | 0.47 | 0.71 |
| Total | 973 ± 133 | 864 ± 114 | 785 ± 144 | 574 ± 70.2 | 2.02 | 0.14 |
| Sulfatide | ||||||
| C16:0 | 6.72 ± 0.81 | 3.76 ± 1.04* | 5.65 ± 0.93 | 2.79 ± 0.29** | 4.72 | 0.012# |
| C18:0 | 63.4 ± 11.5 | 61.0 ± 14.1 | 53.7 ± 5.25 | 41.3 ± 4.2 | 1.05 | 0.39 |
| C20:0 | 3.83 ± 0.91 | 3.74 ± 0.67 | 3.64 ± 0.74 | 2.21 ± 0.19 | 1.28 | 0.31 |
| C22:0 | 34.3 ± 5.36 | 31.0 ± 6.06 | 29.5 ± 4.77 | 20.4 ± 2.46 | 1.49 | 0.25 |
| C22:0-OH | 25.3 ± 1.77 | 18.1 ± 4.62 | 20.7 ± 3.38 | 12.0 ± 1.55* | 3.21 | 0.045# |
| C22:1 | 13.8 ± 2.81 | 10.9 ± 1.68 | 11.5 ± 2.51 | 7.24 ± 0.84 | 1.66 | 0.21 |
| C22:1-OH | 5.41 ± 0.66 | 4.43 ± 1.09 | 4.63 ± 1.15 | 3.34 ± 0.42 | 0.94 | 0.44 |
| C24:0 | 71.0 ± 9.00 | 58.7 ± 14.7 | 61.6 ± 7.38 | 40.4 ± 4.85 | 1.75 | 0.19 |
| C24:0-OH | 16.5 ± 2.05 | 13.2 ± 2.61 | 15.0 ± 2.24 | 10.2 ± 1.51 | 1.61 | 0.22 |
| C24:1 | 320 ± 54.1 | 239 ± 42.0 | 218 ± 38.5 | 148 ± 14.2* | 3.14 | 0.048# |
| C24:1-OH | 21.8 ± 3.39 | 16.8 ± 3.44 | 17.4 ± 3.29 | 12.7 ± 1.14 | 1.57 | 0.23 |
| Total | 582 ± 91.0 | 461 ± 90.0 | 441 ± 66.5 | 300 ± 22.5 | 2.49 | 0.089 |
Levels of S1P, sphingosine, and other sphingolipids in the hippocampus of WT, SK2Δ, J20, and J20.SK2Δ mice at 8 months of age. All lipids shown have the common 18:1 sphingosine base. F and p values for one-way ANOVA are indicated; #p < 0.05. After adjusting ANOVA p values for multiple comparisons, sphingosine and S1P were significant at Q < 0.05, indicated by bold font. Dunnett's post-test was used to compare group means to the WT for individual lipids significant at p < 0.05 in univariate ANOVA.
*p < 0.05,
**p < 0.01.