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. 2019 Nov 26;10:346. doi: 10.1186/s13287-019-1434-3

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — a Alcian blue staining. Cartilage matrix including acidic sulfated mucosubstances and hyaluronic acid are stained in blue. b Quantification of blue matrix showed significantly higher percentages of blue matrix in the LBMP2/GFP-transduced cells compared to non-transduced hMDSCs. Scale bar = 100 μm. c Raman spectroscopy quantification indicated significantly higher amount of sulfate cartilage matrix (proteoglycan aggrecan) in LBMP2/GFP-transduced hMDSCs than in non-transduced hMDSCs. d Raman spectroscopy indicated higher collagen content in the LBMP2/GFP-transduced hMDSCs, although differences were not always significant. e Collagen 2A1 (Col2A1) immunohistochemistry indicated stronger Col2A1 staining in the LBMP2/GFP-transduced hMDSCs compared to respective non-transduced hMDSCs. Scale bar = 100 μm. *P < 0.05, **P < 0.01, ***P < 0.001