Fig. 2.

Downregulation of DLX6-AS1 inhibits NB cell proliferation, migration and invasion. a Relative expression levels of DLX6-AS1 in SK‐N‐SH and SH‐SY5Y cells after transfection with siNC or siDLX6-AS1 was examined by qRT‐PCR (24 h). Knockdown of siDLX6-AS1 markedly inhibited cell b viability and c proliferation in SK‐N‐SH and SH‐SY5Y cells detected by MTT assay (1–5 days) and colony formation assay (2 weeks). d Silenced DLX6-AS1 suppressed the cell migratory capacities of SK‐N‐SH and SH‐SY5Y cells as determined by a wound‐healing assay (24 h). e The results of the transwell assay (24 h) suggested that knockdown of DLX6-AS1 obviously inhibited the capacities of cell invasion in SK‐N‐SH and SH‐SY5Y cells. f Flow cytometry analysis (48 h) was conducted to analyze the apoptosis rates in SK‐N‐SH and SH‐SY5Y cells after transfection with siNC or siDLX6-AS1. g The protein levels of neuronal differentiation markers GAP43 and NF-200 were detected in SK‐N‐SH and SH‐SY5Y cells after transfection with siNC or siDLX6-AS1 by western blot (48 h). *p < 0.05 and **p < 0.01