Fig. 3.

DLX6-AS1 can bind with miR-107 and knockdown of miR-107 attenuates siDLX6-AS1 inhibited effects on NB cell proliferation, migration and invasion. a The predicted binding sites of miR‐107 to the DLX6-AS1 sequence. b Luciferase reporter assays were performed for the detection of the luciferase activities of HEK‐293T cells after transfections. The qRT‐PCR results of miR-107 expression in c NB tissues and d NB cells. e Spearman’s correlation curve indicated a negative correlation between DLX6-AS1 and miR‐107 in NB tissues. f The relative miR‐107 expression in SK‐N‐SH and SH‐SY5Y cells after transfection with siNC, siDLX6-AS1, inhibitor NC or miR‐107 inhibitor was tested by qRT‐PCR analysis (24 h). g MTT assay (1–5 days) and h colony formation assay (2 weeks) results showed cell viability and cell proliferation abilities in two NB cells in different groups. Cell migration and invasion capacities in two NB cells with different transfections were determined by i wound healing assay (24 h) and j transwell assays (24 h), respectively. k Flow cytometry analysis (48 h) was conducted to analyze the apoptosis rates in SK‐N‐SH and SH‐SY5Y cells in different group. *p < 0.05, **p < 0.01 and ***p < 0.001