Fig. 4.

BDNF is the target of miR-107 regulated by DLX6-AS1 and the role of DLX6-AS1/miR‐107/BDNF axis in NB progression. a The predicted binding sites of BDNF to the miR‐107 sequence. b Luciferase reporter assay in HEK‐293T cells was used to confirm the binding between miR‐107 and BDNF. Spearman’s correlation analysis uncovered the association between BDNF expression and c miR‐107 or d DLX6-AS1 level in NB tissues. e Relative BDNF expression in SK‐N‐SH and SH‐SY5Y cells transfected with siNC, siDLX6-AS1, mimic NC, miR-107 mimics, inhibitor NC or miR‐107 inhibitor was tested by qRT‐PCR (24 h) and western blot (48 h). f Western blot analysis (48 h) was applied to evaluate the protein level of BDNF expression in pcDNA3.1 or pcDNA-BDNF transfected SK‐N‐SH and SH‐SY5Y cells. g MTT assay (1–5 days) and h colony formation assay (2 weeks) results showed cell viability and cell proliferation abilities in two NB cells in different group. Cell migration and invasion capacities in two NB cells with different transfections were determined by i wound healing assay (24 h) and j transwell assays (24 h), respectively. *p < 0.05, **p < 0.01 and ***p < 0.001