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. 2019 Nov 27;16:46. doi: 10.1186/s12989-019-0322-4

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Internalization of AgNPs and intracellular soluble Ag-components in AgNPs-treated HepG2 cells. a Cellular delivered dose of AgNPs at 2, 4 or 8 μg/mL after 24 h-treatment (n = 3). b Soluble Ag-components of HepG2 cells treated with AgNPs at 0.6, 1, 2, 4 or 10 μg/mL for 24 h (n = 3). c Ag content of RNA, DNA and protein components in AgNPs-treated HepG2 cells at 2, 4 or 8 μg/mL for 24 h (n = 5). d Chromatograms of Ag-protein complexes from untreated and AgNPs-treated HepG2 cells at 1, 2 or 4 μg/mL for 24 h analyzed using SEC-ICP-MS. e Normalized HMW and LMW fractions from AgNPs-treated HepG2 cells at 0.01, 0.1, 0.2, 0.6, 1, 2 or 4 μg/mL for 24 h analyzed using SEC-ICP-MS (n = 3)