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. 2019 Nov 26;16:234. doi: 10.1186/s12974-019-1634-x

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Differential expression of apoptosis-related proteins in TLR4 knockout mice and wild-type mice after ICH induction. a The expression of Caspase 3, 8, 9, Bcl-1 and Bax in the brain tissue around the bleeding site of TLR4 knockout mice and wild-type mice on days 1, 3, and 7 after ICH induction. b Quantification of caspase 9. c Quantification of caspase 8. d Quantification of caspase 3. e Quantification of Bax. f Quantification of Bcl-2. Western total protein was 30 μg. GAPDH is used as a housekeeping protein to prove the equal loading in each lane of the electrophoresis and normalize densitometric values of the other protein analyzed. Each experiment was repeated three times. *P < 0.05 represents a statistically significant difference between the two groups