Fig. 6.

Inhibitory effect of anti-CD47 and anti-PD-L1 targeting on PDAC tumor growth. a, b Panc02 cells or MPC-83 cells were subcutaneously transplanted into C57BL/6 mice or KM mice to establish pancreatic tumors. CD47 and PD-L1 expression levels on Panc02 and MPC-83 cells from xenografts (in vivo) as measured by immunoblotting (a) and immunofluorescence (b). c Panc02 cells were transplanted to C57BL/6 mice. When the tumor reached 100 mm³, tumor-bearing mice were divided into four groups and treated with anti-CD47 and anti-PD-L1 antibodies, either alone or in combination for 14 days. The tumors removed from each group are shown in the left panel; tumor volume (middle panel) and weight (right panel) were compared to those of the untreated control. p values were calculated based on a Student’s t test. d MPC-83 cells were transplanted to KM mice. When the tumor reached 100 mm³, tumor-bearing mice were treated as in c. The tumors removed from each group are shown in the left panel; tumor volume (middle panel) and weight (right panel) were compared to those of the untreated control. p values were calculated based on a Student’s t test