Fig. 2.

Expression of CD103 on pulmonary CD4⁺ T cells and CD8⁺ T cells. Mice were percutaneous infected by S. japonicum. 5–6 week later, lungs were picked out. Single cell suspensions of lungs were isolated from naive and infected mice. (a) The percentage of CD4⁺ and CD8⁺ T cells in the isolated lymphocytes were detected by FACS. (b,c) The percentage and absolute number of CD4⁺ and CD8⁺ T cells were compared between naive anf infected mice. The expression of CD103 was detected using cell surface staining. The expression of CD103 on CD3⁺, CD4⁺, and CD8⁺ T cells was analyzed using FCM, respectively. (d) A representative result was shown. (e) Average expression of CD103 was calculated from FACS data. (f) To dynamical observe the expression of CD103 in pulmonary T cells, mouse was sacrificed fron week 1 to week 7 after percutaneous infected by S. japonicum. Single cell suspensions of lungs were isolated from naive and infected mice. The average expression level of CD103 on CD4⁺ and CD8⁺ T cells were calculated from FACS data. 5 mice per group and results were shown as mean ± SEM. Variable of the data was analyzed by Tukey’s HSD.* for P < 0.05