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. 2019 Nov 20;13:1262. doi: 10.3389/fnins.2019.01262

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Copyright © 2019 Nässel, Zandawala, Kawada and Satake.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phylogeny of tachykinin and natalisin receptors. RYamide and luqin receptors were used as outgroup to root the tree. Amino acid sequences of full-length receptors were used for the analysis. Sequences were aligned using the MAFFT (E-INS-i algorithm and BLOSUM30 scoring matrix) and the phylogenetic tree constructed using the FastTree plugin in Geneious Prime (2019). Receptors that have been functionally characterized are indicated by a symbol before the species name. Note that in another annelid, Urechis unicinctus (among Lophotrochozoans), the TK receptor has been functionally characterized (not shown here). The figure was constructed in MEGAX. Sequences used to generate the phylogeny are provided in Supplementary Material Text File S1.