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MiR-149 and FoxO3 were involved in myocardial I/R injure. (A) Relative miRNA levels of miR-149a in H9C2 cells. (B–D) mRNA and protein levels of FoxO3 in H9C2 cells subjected to I/R by using RT-PCR and western blotting (E) The expression of FoxO3 in I/R-treated H9C2 cells measured by immunofluorescence stain. The data are presented as the mean±SD, n=3; ** P<0.01 versus control. I/R – ischemia/reperfusion; RT-PCR – real-time polymerase chain reaction; SD – standard deviation.
