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. 2019 Nov 19;25:8733–8743. doi: 10.12659/MSM.918410

Table 1.

Primer sequences for RT-PCR analysis.

cDNA Forward primer Reverse primer
miR-149 5′-CCCTCACTTCTGTGCCAC-3′ 5′- GTGCAGGGTCCGAGGT-3′
FoxO3 5′- CTGGACGGAGTAGCTCCA AG-3′ 5′ - TCAGCATCTCCTCGGTCTCT-3′
NLRP3 5′-GAAAACGGATCCAGATGAAGATGGCA-3′ 5′-TTCCACTCGAGCCAAGAAGGCTCAAAGACG-3′
Caspase-1 5′- ATCCACGAGCAGAGTCAAAG-3′ 5′- CCTGGTCAACTGTCACAAAAC-3′
IL-1β 5′-CCTTGTCGAGAATGGGCAGT- 3′ 5′- TTCTGTCGACAATGCTGCCT- 3′
IL-18 5′-TACTACCGAAACTTGGACC -3′ 5′-CTCAAACTACCTTCTCCG- 3′
U6 5′-CTCGCTTCGGCAGCACA-3′ 5′-AACGCTTCACGAATTTGCGT-3′
β-actin 5′-ACAACTTTGGTATCGTGGAAGG-3′ 5′-GCCATCACGCCACAGTTTC-3′

RT-PCR – real-time polymerase chain reaction; IL – interleukin.