Table 1.
Microscopy observations of the effect of Ab on epithelial cells at different MOIs
| Co-culture time point | Control group a | Experimental group b | |||
|---|---|---|---|---|---|
| MOI 1 | MOI 10 | MOI 50 | MOI 100 | ||
| 2 h | + / - | + (−) / ±(−) | + (−) / ++ (−) | + (+) / & (+) | + (+) / & (+) |
| 4 h | ++ / - | ++ (−) / + (−) | ++ (±) / +++ (−) | ++ (++) / && (++) | ++ (++) / && (++) |
| 6 h | +++ / - | +++ (−) / ++ (−) | +++ (+) / ++++ (−) | +++ (+++) / &&& (+++) | +++ (+++) / &&& (+++) |
| 8 h | ++++ / - | ++++ (−) / ++ (−) | ++++ (+) / ++++ (±) | +++ (+++) / &&&& (+++) | +++ (+++) / &&&& (+++) |
Control: 0.45% NaCl
a: epithelial cells / bacteria; b: epithelial cells (bubble-like dead cells) / bacteria (bacterial aggregation phenomenon)
+: indicated the degree (the epithelial cells were expressed as the situation of adherent growth, bacteria were expressed as quantity, bubble-like dead cells and bacterial aggregation phenomenon were expressed as visibility); &: indicated the degree > ++++; −: indicated none
For example: + / - indicated that a few of epithelial cells adhered to plates without bacteria; +++ (+++) / &&& (+++) indicated that the bacterial number increased and the bacterial aggregation phenomenon became more obvious compared to 4 h, a lot of epithelial cells adhered to plates and the bubble-like dead cells with bacterial adhesion visible