Skip to main content
. 2019 Nov 20;10:778. doi: 10.3389/fendo.2019.00778

Figure 2.

Figure 2

Excessive iodine-induced pyroptosis in TFCs is related to ROS-NF-κB signaling. Nthy-ori 3-1 cells were treated with NaI for 24 h. (A–C) The intracellular reactive oxygen species (ROS) levels were detected by flow cytometry (FCM) analysis and are presented as the positive cells (%) and mean fluorescence intensity (MFI). (D) The intracellular ROS levels, as indicated by DCF fluorescence, were analyzed immediately using immunofluorescence (200×; scale bars, 50 μm). (E) The intracellular ROS levels using NaI with or without N-acetyl-cysteine (NAC) (10 mM) treatment were detected by FCM, and representative FCM graphs of the ROS-positive cells (%) and mean fluorescence intensity (MFI) are shown. (F,G) Nthy-ori3-1 cells were treated with NaI at different time points, and the p65 and p-p65 expression levels were measured by immunoblots. (H,I) Nthy-ori3-1 cells were treated with NaI at 24 h in the presence or absence of NAC, and p65 and p-p65 expression levels were measured by immunoblots. (J–L) Nthy-ori 3-1 cells were treated with NaI and/or NAC and/or IKK-16 for 24 h, and GSDMD expression levels were measured by immunoblots. All statistical results shown are representative of three replicates. Significant differences and P-values were calculated by one-way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001.