Figure 4.

Excessive iodine-induced pyroptosis activation contributes to IL-1β secretion in TFCs. (A,B) Representative results of interleukin-1β (IL-1β) immunohistochemical staining in HT tissues (n = 20) and control tissues (n = 10) are shown. Brown regions represent positive expression (original magnification, ×200; scale bars, 100 μm). (C) IL-1β levels in the supernatants of Nthy-ori 3-1 cell cultures were detected by ELISA in the presence of NaI treatment, with or without NAC, IKK-16, and silencing of NLRP3 at 24 h. The statistical results shown are representative of three replicates. Significant differences and P-values were calculated by unpaired t-tests or one-way ANOVA. *P < 0.05, ***P <0.001.