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. 2019 Oct;189(10):1945–1952. doi: 10.1016/j.ajpath.2019.06.004

Figure 2.

Figure 2

Up-regulation of lysyl oxidase propeptide (LOX-PP) compromised AKT activity in endothelial cells from rat retinas (RRECs). A: Representative Western blot image shows protein expression of phospho- (p-)AKT, total AKT, cleaved caspase-3, and β-actin in RRECs grown in normal (N) medium, high glucose (HG) medium, N medium exposed to recombinant (r)LOX-PP (N+rLOX-PP), or N medium exposed to mannitol (N+Man). B: Graphical illustration of cumulative data shows up-regulation of LOX-PP alone compromised AKT activation in RRECs. Cells exposed to mannitol did not exhibit changes in AKT activity. C: Graphical illustration of cumulative data shows cleaved caspase-3 expression was significantly increased in cells grown in HG and in cells grown in N medium exposed to rLOX-PP. No significant change in cleaved caspase-3 activity was observed in cells exposed to mannitol. Data are expressed as means ± SD. *P < 0.05 versus N.