Supplemental Figure S4.

The in vitro decellularized extracellular matrix (ECM) model mimics key features of fibrotic ECM in obesity. A: Composite and single-channel confocal microscopy images of ECM, assembled by lean and obese adipose stromal cells (ASCs). B–D: Decellularization does not affect the intrinsic fibrillar structure of ECMs regardless of ASC source (B) but recapitulates increased fibronectin and collagen I content in ECMs assembled by obese versus lean ASCs (C and D), as determined by immunofluorescence image analysis. Data are expressed as means ± SD (C and D). n = 1 representative experiment (C and D). **P < 0.01 (t-test analysis). Scale bars = 25 μm (A and B).