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. 2019 Oct;189(10):2019–2035. doi: 10.1016/j.ajpath.2019.06.005

Figure 3.

Figure 3

Macrophage phenotype is associated with obesity-associated interstitial fibrosis. A and B: Interstitial extracellular matrix is more fibrotic in obese versus lean or overweight breast adipose tissue, as determined by image analysis and pixel quantification of picrosirius red–stained sections under polarized light. Kruskal-Wallis analysis was used. C–F: Interstitial fibrosis positively correlates to CD206+ immunohistochemistry (IHC) and CIBERSORT anti-inflammatory (M2) score (C and D) but not CD11c IHC (E) or CIBERSORT pro-inflammatory (M1) (F) score. C–F: Red dotted lines illustrate the best fit line, as determined by linear regression. Data are expressed as median and interquartile range (B). n = 37 with 5 representative images per section (A and B); n = 37 (C, D, and F); n = 26 (E). **P < 0.01. Scale bars = 50 μm (A).