Table 1:
Component | Stock concentration | Final concentration | Dilution | Amount to add (µL) |
---|---|---|---|---|
1. Cleavable picolyl biotin azide | 10 mM | 100 µM | 100 | n * v * 1.2 /100 |
2. Copper sulfate | 50 mM | 250 µM | 200 | n * v * 1.2 /200 |
3. THPTA | 10 mM | 250 µM | 40 | n * v * 1.2 /40 |
4. Sodium ascorbate | 50 mM | 2.5 mM | 20 | n * v * 1.2 /20 |