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. Author manuscript; available in PMC: 2020 Dec 1.
Published in final edited form as: Curr Protoc Chem Biol. 2019 Dec;11(4):e75. doi: 10.1002/cpch.75

Table 1:

Calculation of reagent amounts for CuAAC reaction master mix. Volume per sample (µL) = v; number of samples = n. Volume of master mix to add to each sample = 0.1 * v (i. e. for a 250 µL sample add 25 µL of master mix)

Component Stock concentration Final concentration Dilution Amount to add (µL)
1. Cleavable picolyl biotin azide 10 mM 100 µM 100 n * v * 1.2 /100
2. Copper sulfate 50 mM 250 µM 200 n * v * 1.2 /200
3. THPTA 10 mM 250 µM 40 n * v * 1.2 /40
4. Sodium ascorbate 50 mM 2.5 mM 20 n * v * 1.2 /20
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