Figure 8.

Percentage cell viability using MTT assay on the MCF-7 cell line. The cells were treated with the following formulations for 48 h: CuFe₂O₄ (A), HYPS (B), CuFe₂O₄/HYPS (C), cisplatin (D), and cisplatin/CuFe₂O₄/HYPS (E). The treatment concentrations used were as indicated in the figure. The reason for the different concentrations of CuFe₂O₄ and cisplatin is because they correspond to the amount adsorbed on the cisplatin/CuFe₂O₄/HYPS nanoparticles. For details, please see the Materials and Methods section. n = 4 independent experiments. Dashed line represents untreated control. Error bars ± standard error of the mean (SEM) and * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001 versus control.