Skip to main content
. Author manuscript; available in PMC: 2019 Nov 27.
Published in final edited form as: ACS Nano. 2019 May 29;13(6):6670–6688. doi: 10.1021/acsnano.9b01004

Figure 2.

Figure 2.

MSC-derived exosome-treated mice showed a reduced number of infiltrated macrophages/microglia and T cells in the spinal cords of EAE mice. (A) Immunohistochemistry of Iba-1, a Ca2+-binding protein indicative of macrophages and microglia, and the nuclear stain DAPI in spinal cord sections of EAE mice (Left and middle panel scale: 400 μm; right panel scale: 80 μm). Yellow inset frames are magnified images showing the morphology of macrophages. (B) Quantification of Iba-1 positive cells from immunohistochemistry images. Iba-1 positive cells were counted in the spinal cords’ sections in both white and gray matter areas (n = 3; **p < 0.01; ***p < 0.001). (C) Representative flow cytometry plots of CD4+ and CD8+ T cells within spinal cords. (D) and (E) Flow cytometry analysis of spinal cords stained for infiltrated CD4+ and CD8+ T-cells, respectively. Unpaired t tests were used to determine p values (n = 3; *p < 0.05; **p < 0.01).