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. 2019 Nov 27;14(11):e0225584. doi: 10.1371/journal.pone.0225584

Fig 1. The inhibitory effects of polypyrrole on the biofilm formation of S. mutans.

Fig 1

Effects of various concentrations of polypyrrole on the biofilm formation of S. mutans UA159 were observed on human saliva–coated 96-well microtiter plates after incubation for 16 h in TSB supplemented with 0.25% sucrose. A: Biofilm formation in S. mutans was present at high concentrations of polypyrrole (63, 125, 250 and 500 x 10 μg/ml) on human saliva–coated 96-well microtiter plates. B: Biofilm formation in S. mutans UA159 was quantitatively analyzed at high concentrations of polypyrrole. C: Biofilm formation in S. mutans UA159 and MT8148 was quantitatively analyzed at various concentrations of polypyrrole. D: Biofilm formation in the S. mutans strains GS-5 and MT8148, clinical isolates FSC-8, FSM-5, and FSC-7, and S. sobrinus AHT and OMZ176 were quantitatively analyzed at 2.5 mg/ml polypyrrole. The data indicated the mean ± SD of triplicate experiments. The independent experiments were performed 3 times, with similar results obtained in each. The asterisks indicated a significant difference between 2 groups (*: p < 0.05, no polypyrrole vs polypyrrole).