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Cytosolic proteins were collected from FB1 wild-type U. maydis and from a mutant deficient in either O- or N- glycosylation upon Biz1 repression or induction conditions. O- or N-glycoproteins are purified through a Concanavalin A FPLC column using O- or N-glycoprotein specific eluents. Finally, glycoproteins are tagged with fluorophores and separated by 2D electrophoresis.
