Fig 3. Mucin production in T. muris trickle infection.

(A) Relative expression of goblet cell-associated genes measured by qPCR. Relative quantification was achieved using the 2^-ΔΔCT method with β-actin used as the housekeeping reference gene. (B) Western blot to visualize Muc5ac protein from extracted mucus from naïve or T. muris infected C57BL/6 mice at 11 weeks p.i.. (C) Quantification of the mean number of Muc2+ goblet cells per crypt, given as the mean number of Muc2+ cells from a minimum of 10 crypts per mouse. Representative immunostained sections during trickle infection. Muc2 in green. DAPI stain in blue. n = 5, statistical analysis completed by a one way-ANOVA comparing each time point to week 0. Data presented as mean +/- SEM, * = p<0.05.