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. 2019 Nov 15;15(11):e1007863. doi: 10.1371/journal.ppat.1007863

Fig 1. In vitro characterization of constructed EV-A71 variants.

Fig 1

(A) Schematic illustration of the EV-A71 genome and the infectious clone constructs. Different amino acids were substituted at VP2-149, VP1-98 and VP1-145 (labeled in bold), with reference to the wild type (WT) strain KEQ. (B) The clone-derived EV-A71 variants were propagated in RD cells and showed comparable plaque morphologies (C) The binding affinity of EV-A71 variants to heparin sepharose beads was analyzed. (D) Inhibitory effect of heparin on EV-A71 variants was evaluated by pretreating the viruses with soluble heparin before infection of RD cells. Results are presented as mean ± SD (n = 3). Error bars indicate standard deviations from triplicates. Statistical significances are denoted with *P < 0.05, **P < 0.01 as compared to the WT.