Fig 4. Viral load quantitation from harvested organs and viremia level induced by EV-A71 variants.

(A) At selected time points, IEQ, IEE and IEQ-244E⁺-infected mice (n = 5) were sacrificed and viral loads were determined from harvested hind limbs and brains using qRT-PCR. Significant differences between viral variants are labelled as ** (P < 0.01) and *** (P < 0.001). (B) Virus clearance from blood was quantitated using qRT-PCR following intravenous inoculation of KEQ, KKE, KEE, IEE, IEQ or IEQ-244E⁺ into 3–4 weeks old mice. The viral RNA copies were quantitated at selected timepoints up to 30 minutes. The data are presented as V_t/V₀, indicating the fraction of viral RNA copies at each timepoint (V_t) over the initial viral RNA copies (V₀). Significant differences between KEQ and IEQ-244E⁺ are labelled as * (P < 0.05) and ** (P < 0.01).