Figure 1.

Phenotype Analysis of wp1 Flowers.

(A) to (C) Flowers of the wild type (A), wp1-1 (B), and wp1-2 (C). Bars = 1.5 mm.

(D) to (F) Dissected flowers of wild type. The vexillum petal (D), the fused alae and keel petals (E), and the stamens (F) of the wild type. Bars = 1.5 mm.

(G) to (I) Dissected flowers of wp1-1. The vexillum petal (G), the fused alae and keel petals (H), and the stamens (I) of wp1-1. Bars = 1.5 mm.

(J) HPLC chromatograms and characteristic absorbance spectra of the extracted and saponified carotenoids from petals of the wild type and two wp1 mutants. The peaks for lutein, lutein epoxide, and violaxanthin are marked. Insets are spectra of indicated peaks. mAU, milli-absorbance unit; WT, wild type.

(K) Contents of lutein, lutein epoxide, and violaxanthin in petals of the wild type and two wp1 mutants. Bars represent means ± sd of three biological replicates. Asterisks indicate differences from the wild type (**P < 0.01, Student’s t test). FW, fresh weight; WT, wild type.