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. 2019 Sep 17;31(11):2751–2767. doi: 10.1105/tpc.19.00480

Figure 2.

Figure 2.

Molecular Cloning and Confirmation of the WP1 Gene.

(A) Schematic representation of the gene structure of WP1 showing the Tnt1 insertion sites in wp1-1 and wp1-2. Introns are represented by a line, and exons are represented by a striped box.

(B) RT-PCR showing transcript abundance of WP1 in the flowers of the wild type and wp1 mutants. MtActin was used as the control. WT, wild type.

(C) Genetic complementation of wp1-1. Representative flowers of the wild type, wp1-1, and wp1-1 complemented with WP1-GFP (WP1-GFP/wp1-1). Bars = 1.5 mm. WT, wild type.

(D) Transcript levels of WP1 in petals of the wild type, wp1-1, and WP1-GFP/wp1-1. Bars represent means ± sd of three biological replicates. Asterisks indicate differences from the wild type (**P < 0.01, Student’s t test). WT, wild type.

(E) Lutein concentrations in petals of the wild type, wp1-1, and WP1-GFP/wp1-1. Bars represent means ± sd of three biological replicates. Asterisks indicate differences from the wild type (**P < 0.01, Student’s t test). WT, wild type.