LPCAT Activity in Isolated Chloroplasts.
Isolated chloroplasts from Col-0, act1, lpcat1 lpcat2, and act1 lpcat1 lpcat2 were incubated with 1 mM soy LPC, and 13.6 µM [14C]oleoyl-CoA for 30 min at 30°C and radioactivity incorporated into PC measured. The data represent the average and se of three independent assays from chloroplasts isolated from each line. Significant (p-value < 0.05, Student’s t test) differences from the Col-0 control are indicated by asterisks above the bars.