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. Author manuscript; available in PMC: 2019 Nov 27.
Published in final edited form as: ACS Appl Mater Interfaces. 2019 Feb 21;11(9):8849–8857. doi: 10.1021/acsami.8b21270

Figure 3: Increasing biochemical ligand density leads to increased focal adhesion punctae formation regardless of matrix stiffnesses.

Figure 3:

hMSCs were cultured on soft and stiff substrates with low or high ligand densities. (a) Immunostaining of pFAK, a focal adhesion marker;(b) immunostaining of paxillin (green) and vinculin (red); (c) quantification of focal adhesion punctae; and (d) immunostainings of β1- and αVβ3- integrins; (e) PCR array analyses of genes related to focal adhesion expressed by hMSCs cultured on stiff substrates at low and high fibronectin densities. Scale bars, 30 μm.