(a) Distributions of FFAT scores in human and yeast (S.
cerevisiae). FFAT scores are assigned as described in Methods. The means and standard deviations
are yeast: 4.6±1.1, human 4.5±1.1; the distributions are
statistically similar (Chi2 0.998). B. Proportion of 6
eukaryotic proteomes at different levels of low FFAT score, from highly optimal
(top, range 0.0 – 1.0) to less optimal (bottom, =2.5), including the
average, un-weighted for number of proteins in each proteome, and standard
deviation. C. Proportions of yeast and human proteins with motifs
with highly optimal FFAT scores (range 0.0 – 1.0) showing if they are
known interactors of VAP. D. Proportion of proteins identified as
binding VAP in yeast and human according to their FFAT scores (range 0.0
– 6.0). Inset shows detail in the range FFAT scores 1.5 – 2.5.
Dotted lines indicate the average across the two species. Identification of
proteins as VAP interactors declines rapidly as FFAT score increases: 59% for
FFAT score ≤1.0 (human 11/17, yeast 2/5); 8% for
1.0<FFAT score≤2.0 (human 17/180, yeast 3/62); and 3% for
2.0<FFAT score≤3.0 (human 11/440, yeast 5/146).