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. Author manuscript; available in PMC: 2020 Apr 21.
Published in final edited form as: Nat Microbiol. 2019 Oct 21;4(12):2393–2404. doi: 10.1038/s41564-019-0590-7

Figure 5. RUMGNA_02695 catalyses the conversion of 2,7-anhydro-Neu5Ac to Neu5Ac.

Figure 5

a) High Performance Liquid Chromatography (HPLC) expand acronyms number replicates with same outcome analysis of DMB labelled RUMGNA_02695 reactions with 2,7-anhydro-Neu5Ac using different co-factors. NAD (black), NADH (pink), FAD (blue), no co-factor (brown), and a Neu5Ac standard (green), data is representative of five independent experiments. b) Michaelis-Menten plot of the rate of reaction for RUMGNA_02695 with increasing concentration of 2,7-anhydro-Neu5Ac. The rate of reaction (μM NADH) at each concentration was determined in triplicate by measuring A340nm change and using a standard curve, the mean value is plotted with standard error of the meaning shown with error bars