Figure 2.

Snapshots from the online resource for GPCR crystallography and cryo-EM. Construct design tool: The Construct design tool allows generation of complete structure constructs for any human GPCR based on data on all available structures and stabilising mutations. The first section (left) presents three application modes: Truncation/Fusion scan, Mutation scan and Custom constructs; the snake plot (right) maps construct modifications onto a 2D sequence view; and the table (bottom) lists all designed constructs and their modifications. Structure view: The single-structure entry view (accessible from the Construct alignments) details all protein engineering, methods and reagents used to determine a given structure. Construct alignments: The Construct alignments has four –Browser, Wild type, Construct, and Sequence– alternative views that can be combined to compare the modifications in any set of constructs, as well as the structure determination method, resolution and release date. Experiment browser: The Experiment Browser tabulates methods and reagents for protein expression, purification, and structure determination from GPCR structure publications. Truncation, fusion and loop deletion sites: These pages visualise the frequencies for the specific truncation, fusion and loop deletion sites used in all structures, organised by receptor and class. Stabilising mutation analyser: The Stabilising Mutation Analyser shows stabilising mutations along with e.g. sequence conservation, helix propensity, hydrophobicity or functional receptor sites. Mutation browser: The Mutation browser shows all mutations and their reported effects in constructs for structural determination. Mutation substitution matrix: The Mutation substitution matrix shows the frequency of amino acid pairwise substitutions across all unique constructs for structural determination.