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. 2019 Nov 26;39(11):BSR20182376. doi: 10.1042/BSR20182376

Figure 6. LINC01296 competitively binds to miR-143-3p to down-regulate MSI2 and inhibit the AKT/STAT3 signaling pathway.

Figure 6

BHT-101 and IHH4 cells were treated with si-NC, si-MSI2, si-LINC01296, NC-mimic, miR-143-3p mimic, si-LINC01296 + miR-143-3p inhibitor or miR-143-3p mimic + pcDNA-MSI2. (A) The protein expression of AKT and STAT3 as well as the extents of AKT and STAT3 phosphorylation in BHT-101 cells normalized to GAPDH determined by Western blot analysis; (B) quantification of results from (A); (C) the protein expression of AKT and STAT3 as well as the extents of AKT and STAT3 phosphorylation in IHH4 cells normalized to GAPDH determined by Western blot analysis; (D) quantification of results from (C). #, P<0.05, compared with the NC-mimic group; *, P<0.05, compared with the si-NC group; the measurement data were expressed as mean ± standard deviation; one-way analysis of variance was used for comparison among multiple groups; the experiment was repeated three times.