Fig. 4.

Binding affinity of IDD10 for the promoters of CIPK9 and CIPK14. (A) Schematic diagram showing the locations of putative IDD10-binding motifs in 1.5 kb regions of the promoters of five different CIPK genes. The areas amplified by the probes P1–P16 in the ChIP assay are underlined. (B) Results of the ChIP assay. DNA was immunoprecipitated and amplified to determine the extent to which IDD10 bound regions P1–P16 within a 1.5 kb portion of each CIPK promoter. The relative ratios of immunoprecipitated DNA to input DNA were determined using qPCR. Input DNA was used to normalize the data. DNA immunoprecipitated without addition of GFP antibody (Ab) was used as a control. Data are means ± s.e. (n = 3); different letters indicate significant differences between results (P < 0.05).