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. 2019 Nov 21;7:288. doi: 10.3389/fcell.2019.00288

FIGURE 1.

FIGURE 1

Oxygen-sensitive gene expression in NSCs upon hypoxic exposure. (A) Representative Nestin/Sox2 and Dcx/Tuj1 double stainings of C17.2 cells cultured in standard condition with 21% O2; nuclei were counterstained with DAPI. (B–D) mRNA expression of rbm3 (B), cirp (C), and kdm3a (D) was measured 16 h after ambient normoxic (21% O2) or indicated hypoxic treatment. Actb was used as an internal control. One-way ANOVA followed by Dunnett’s test was used to compare each hypoxic condition to the normoxic group. N.S. not significant; ∗p < 0.05; ∗∗p < 0.01. (E) Representative Western blot, RBM3, and CIRP were measured 24 h after ambient normoxic (21% O2) or moderate hypoxic (2.5% O2) treatment; GAPDH was used as a loading control. (F) Band relative intensity in panel (E) was normalized, and unpaired t test was used for statistical analysis. N.S., not significant; ∗p < 0.05; ∗∗p < 0.01.