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Hypoxia induces cell cycle arrest in NSCs. Cell cycle analysis of C17.2 cells after 24 h 5% O2, 2.5% O2, and 1% O2 hypoxic treatment. Ambient normoxic (21% O2) was used as control. Representative flow cytometry DNA histogram with propidium iodide (PI) staining. One-way ANOVA followed by Dunnett’s test was used for statistical analysis in G0/G1 phase, S phase, and G2/M phase (n = 3). N.S., not significant; ∗∗p < 0.01.
