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. 2019 Nov 21;7:288. doi: 10.3389/fcell.2019.00288

FIGURE 3.

FIGURE 3

RBM3 overexpression increases NSC viability and proliferation under moderate hypoxia. (A) RBM3 protein expression after transient transfection with overexpressing vector. Vec: empty vector; RBM3 OE: RBM3-overexpressing vector. (B) Representative flow cytometry dot plots of transfected C17.2 cells with LIVE/DEAD viability staining after 24 h moderate hypoxia (2.5% O2). Unpaired t test was used for statistical analysis (n = 3). ∗p < 0.05. (C) Representative flow cytometry DNA histogram of transfected C17.2 cells with propidium iodide (PI) staining after 24 h moderate hypoxic (2.5% O2) treatment. Vec: empty vector; RBM3 OE: RBM3-overexpressing vector. Unpaired t test was used for statistical analysis in G0/G1 phase, S phase and G2/M phase (n = 3). N.S., not significant; ∗p < 0.05.