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. 2019 Nov 21;7:288. doi: 10.3389/fcell.2019.00288

FIGURE 4.

FIGURE 4

RBM3 positively regulates proliferation in primary NSC from P0 mice under hypoxia. (A) Representative Nestin/Sox2 and Dcx/Tuj1 double staining of primary RBM3 WT and KO NSCs from the forebrains of P0 mice. Nuclei were counterstained with DAPI. (B) RBM3 protein expression in RBM3 WT and KO primary NSCs, and vector transfected WT NSCs from the forebrains of P0 mice. Vec: empty vector; OE: RBM3-overexpressing vector. (C) Representative BrdU and DAPI staining of P0 WT or KO NSCs after 24 h 21, 5, 2.5, or 1% O2 treatment. (D) Two-way ANOVA followed by Tukey’s test was used for the comparisons in panel (C). N.S., not significant; ∗p < 0.05; ∗∗p < 0.01. (E) Representative BrdU and DAPI staining of P0 WT NSCs transfected with empty or RBM3-overexpressing vector after 24 h 21, 5, 2.5, or 1% O2 treatment. (F) Two-way ANOVA followed by Tukey’s test was used for the comparisons in panel (E). N.S., not significant; ∗p < 0.05; ∗∗p < 0.01.