Figure 2.

Intrinsic control of iNKT cell development by Foxo1. CD45.1⁺CD45.2⁺ WT mice were irradiated with 800 rad X-ray and intravenously injected with a mixture of CD45.1⁺ WT and CD45.2⁺ Foxo1^f/f -Cd2iCre BM cells at a 1:1 ratio. Recipient mice were euthanized and analyzed 2 months after reconstitution. (A) Representative FACS plots showing CD45.1 and CD45.2 staining in splenic Ly6C⁻Ly6G⁺CD11b⁺ neutrophils. (B) Scatter plots showing ratios of CD45.2⁺ to CD45.1⁺ neutrophils. (C) Representative FACS plots showing CD4 and CD8 staining of live gated thymocytes (left panel) and CD45.1 and 45.2 staining of CD4⁺CD8⁺ DP thymocytes (right panel). (D) Representative FACS plot showing CD1dTet and TCRβ staining (left panel) of live gated Lin-thymocytes and CD45.1 and 45.2 staining of gated CD1dTet⁺TCRβ⁺ iNKT cells (top right panel) and CD1dTet⁻TCRβ⁺ non-iNKT cells (bottom right panel). (E) Representative FACS plot showing CD45.1 and 45.2 staining of live gated CD1dTet⁺TCRβ⁺ iNKT cells from the spleen and liver. (F) CD45.2/CD45.1 ratios of indicated populations of cells. Data shown are representative or pooled from five experiments. One chimeric mouse was examined in each experiment.